Host: The Japan Society of Mechanical Engineers
Name : [in Japanese]
Date : October 14, 2023 - October 15, 2023
The protective effects of pressurized argon gas against cell damage during cold storage on a rat heart striated myocytes monolayer were investigated. The cell monolayers incubated in a culture dish for 24 h were prepared as test samples. The samples were pressurized in 0, 0.4, 0.5, or 0.6 MPa in argon gas, and then preserved at 6°C for 24 h. The pressure-dependent cell viability was evaluated using the water-soluble tetrazolium salts assay. The results showed that protective effect in 0.5 and 0.6MPa were higher than that in 0 MPa (no pressurized dissolution of argon gas). And cell viability increased with increasing the argon gas pressure. Therefore, cell damage due to low temperature will be reduced by pressurized dissolution of argon gas in cold storage.
