Whole transcriptome analysis comparison of Aedes aegypti 6- and 14- day post-dengue infection using RNA-seq

Abstract

Dengue virus is the causative agent of fatal disease, which has no vaccine available. Breakage of transmission is still the core of prevention. The prospects of innovating new prevention techniques include genetically modified dengue-resistant transgenic mosquitoes. That innovation begins with a search of mosquito genes that are affected by dengue infection. Using RNA-seq technique by Illumina Genome Analyzer, we have previously analyzed and compared whole transcriptome of 14-day dengue-infected and non-infected Aedes aegypti. At present, we analyzed and compared 6- and 14-day dengue-infected Ae.aegypti. Approximately 70 millions and 60 millions short reads were obtained from 6- and 14-day dengue infected mosquitoes, respectively. They were mapped onto 14,653 and 14,275 genes. Among them, a total of 11 and 14 genes were up- and down-regulated more than 7.39 folds, respectively. We will report the genes that are found to be significantly affected by the interaction between Ae.aegypti and dengue virus.