Abstract
Aspergillus fumigatus produces several toxic secondary metabolites, such as gliotoxin, several toxic secondary metabolites, such as gliotoxin, fumagillin, helvolic acid, and others. Of the toxins detected in the hypha of Aspergillus fumigatus, fumagillin was well-known as an inhibitor of endothelial cell proliferation, tumor-induced angiogenesis, and tumor growth in mice, although it was known for nearly 40 years as a naturally secreted antibiotic of Aspergillus fumigatus used to treat human amoebiasis. In this study, we have tried to reveal the expression of TNF signaling genes by fumagillin in mouse macrophage (RAW264.7 cell) using multiplex PCR (MPCR), because these activity may be represented in both local and systemic inflammatory events during the fungal infection. MPCR data in this study showed that all of the TNF signaling genes, such as COX-2, NFκ-B, Fas, TNF-α, bcl-2, p53, and Iκ-B, were dose-dependently expressed at low concentration of fumagillin. These results suggest that fumagillin can be a critical role in the TNF signaling pathway causing inflammation and apoptosis during the fungal infection. Certainly, more tests and in-depth studies of this process are needed. Nevertheless, this study represents the first step in showing the effect of fumagillin on TNF signaling genes expression.
