Targeting Therapy for Pancreatic Cancer Using Antibody Dependent Cell-mediated Cytotoxicity Mediated by Chimeric Monoclonal Antibody Nd2.

Abstract

Nd2 is murine monoclonal antibody derived from purified mucin of human pancreatic cancer cell line SW1990. We prepared mouse/human chimeric antibody Nd2(cNd2) and reported that cNd2 could induce antibody dependent cell-mediated cytotoxicity(ADCC) in vitro. In this study, we investigated the effect of cNd2 in nude mouse, and the enhancement of ADCC of cNd2 by IL-2 stimulated lymphocytes in vitro. In nude mouse, intraperitoneal injection of cNd2 inhibited the tumor growth in xenografted model, elongated the survival in orthotopically transplanted model, and suppressed the number of liver metastasis in spleno-injected model. In vitro study, IL-2 enhanced ADCC of cNd2.