Abstract
A series of chemically synthesized oligosaccharides have been examined for developing drags which show anti-peritoneal dissemination activities using mouse greater omentum and gastric and colorectal cancer cells. A new ex vivo assay method has also been developed and it could be conveniently used for evaluation of each oligosaccahride with a relatively longer incubation period. Galβ1, 3[3OMeGalβ1, 4GlcNAcβ1, 6] αBn was found to possess the strongest anti-adhesion activity bewteen MKN 45, colon26 cells and greater omentum even though the compound did not show any anti-proliferation activities against those cells at all. Further, the formation of tumors in the greater omentum of Balb/c mice which were intraperitoneally inoculated with colon26 and Galβ [3OMeGalβ 1, 4GlcNAcβ1, 6] GalNAcαBn was observed to be significantly reduced in comparison with that in mice inoculated only with colon26. It must therefore be possible to develop anti-adhesion therapy with this novel oligosaccahride.
