Abstract
We discuss how to acquire logicality, orientation, and connectivity of action potential from living neuronal network in vitro. The action potential of rat hippocampal neurons organized into complex networks is detected in a culture dish by MED system carried with 64 planar microelectrodes. We propose an analysis method to acquire logicality and orientation of action potentials detected at three electrodes of the culture dish by fuzzy operators incorporating t-norm and tconorm operators. In addition, we propose a definition of connectivity of action potentials among electrodes by fuzzy inclusion degree. Finally, we discuss the relationship between logicality and connectivity of action potentials propagating with an orientation, e.g., transmission, absorption, and diffusion. We show that fuzzy concept is useful even in living neuronal network in vitro.
