Abstract
Bone-derived cells can generate calcified afibrillar globular accretions, directly on a polystyrene culture dish in vitro. Cells anchored to the first formed accretions will produce collagen fibers. While being produced, the collagen layer undergoes calcification. Clearly, the calcified globular accretions may act as a substratum conditioning layer prior to overt bone formation. Similar deposits can be seen on the retrieved implants. Most dental implants are designed to perform stabilization and initial anchorage in bone. However, there seems to be no consideration for the effects of surface roughness on mineralized globular accretions formation. In this study, the deposition of calcified globular matrix on differing surface roughness following 3 weeks of culturing was investigated.
Three different degrees of surface roughness were produced on the bottom of 35 mm tissue culture dishes as follows:1)unabraded (smooth surface), 2) abraded the surface using ＃600 silicon carbide sandpaper, and 3)sandblasted the surface. Bone marrow cells obtained from the femora of rat were cultured for 3 weeks in the above dishes. After culturing, these samples were prepared for SEM.
On the smooth-surfaced dishes, mineralized globular matrix was produced homogeneously and densely. However, on the 600-grit-surfaced dishes, mineralized globular accretions were only observed on the top of scratches. Globular accretions could not be seen in the sulcus of scratches. Globular accretions were also observed at the top of pit but not at the bottom of pit.
The results demonstrated that substratum surface roughness influences the distribution of mineralized globular accretions in culture by rat bone marrow derived cells.
