1997 Volume 10 Issue 1 Pages 40-44
Electrolytic acid water (EAW) has a strong bactericidal action (benefit) and severe cytotoxicity (risk). The action ends immediately after clinical use because EAW is restored to the former state of just water. These characteristics should be considered in clinical usage because of being very different from ordinary disinfectants.
In this study, the cytotoxicity of EAW was investigated by tissue culture test using MEM＋10% FBS and bone marrow cells derived from monkey tibia, and the bactericidal action of EAW is tested by MBC of bacterial cultivation in relation to Cl contents in EAW. In addition, the decreasing effects of bovine serum, human saliva, and peptone solution on the bactericidal action of EAW was measured by MBC for MRSA and MSSA.
Cytotoxicity of EAW was 50% less than the EAW/MEM rate. The bactericidal action of EAW was decreased with deactivating effect of Cl and disappeared completely in less than 3 ppm of Cl content. In addition, the bactericidal action of EAW was inhibited by human saliva (5.0% or more), peptone (2.5% or more),and bovine serum (1.3% or more).
The cells of tissue have the same microorganisms as bacteria. Strong bactericidal action usually has high cytotoxicity. These effects mainly depend upon the Cl content in EAW, which is easily influenced by biological substances of blood, tissue fluid, and saliva. EAW should be used with sufficient knowledge of the above mentioned data.
