1997 Volume 10 Issue 4 Pages 454-458
The purpose of this study was to investigate the characteristics of mineralization of new bone tissues in the space created by barrier membrane.Defects were made in the cortical bone of rat tibias. An e-PTFE membrane was applied to the defect and a control defect was not covered with a membrane. Calcein was injected at 6, 8, and 10 days after the experiment and sacrificed at 48 hours after the injection. Villanueva bone satining was performed before observing by confocal laser scanning microscopy. Two labeling styles of mineralization by calcein were detected. One type of labeling was a diffuse type which detected entire new bones diffusely and the other was a peripheral type which detected only the periphery of new bones. At 6 days in both experimental and control groups, only the diffuse type was seen. At 10 days in the experimental group, calcein was detected only periphery. At 10 days in the control group, however, calcein was detected peripherally in the lower portion but diffuse in the upper portion. From these results, it was suggested that the mineralization using the GBR method accelerates the mineralization in both upper and lower portions.
