Abstract
In order to investigate, quantitatively, the adverse effect of electrolytic acid water (EAW) on human gingival mucoperiosteal flap tissue, the relative outgrowth value (ROV) was measured by tissue culture testing with MEM十10%FBS and a CO2 incubator. The tissue fragments were rinsed with and stored in the following nine different conditions:①MEM(Cont.), ②EAW, ③Aqua dest., ④Hanks solution, and ⑤KN solution, and also double treatment of ⑥EAW-Hanks, ⑦EAWKN, ⑧Aqua dest.-Hanks, and ⑨Aqua dest.-KN. After that, ROVs from the explants of mucoperiosteal flap tissue fragments were measured after 14 days of cultivation. In this tissue culture test, it was clearly demonstrated that EAW and Aqua dest. have severe cytotoxicity and an adverse effect on mucoperiosteal flap tissue, while KN solution and Hanks solution have no cytotoxic and adverse effects.
Use of KN solution or Hanks solution is highly recommended as a rinsing solution during mucoperiosteal flap operation in implant surgery. Water-irrigation or EAW desinfection should be avoided, without fail, during implant surgery.
However, post-rinsing with KN solution or Hanks solution may be effective to reduce the adverse effect of EAW or Aqua dest.
