Bone Induction by Human Demineralized Dentin Matrix/Recombinant Human BMP-2 Complex Implant

Abstract

A human dentin after teeth extraction was demineralized for recycle use, and its efficacy as a carrier matrix for recombinant human bone morphogenetic protein-2(rhBMP-2) was evaluated histomorphometrically. The shape of demineralized dentin matrix (DDM) was prepared as a particle, and its size varied from 400 to 800μm. In 50 male Wistar rats, the dose-response and time-course of bone induction by rhBMP-2, in 70 mg of DDM in subcutaneous tissue, was estimated. The rhBMP-2/DDM induced bone and cartilage in a dose dependent manner (0, 0.5, 1.0, 2.0, 5.0μg of rhBMP-2). Histological examination in the time‐course study showed that the BMP-2(5.0μg)/DDM (70 mg) induced active bone and cartilage formation between particles of DDM, while the DDM (70 mg) alone resulted in the accumulation of undifferentiated mesenchymal cells and fibroblasts. The morphometric analysis demonstrated that the BMP‐2/DDM showed 66.9% in the volume of bone and marrow, and 32.4% in that of DDM at 8 weeks.
These results indicate that the DDM derived from extracted human teeth may be recycled as a carrier matrix for rhBMP‐2. It provides a cell‐anchorage for differentiation of chondro‐osteogenic cells and is absorbed as bone matures.