Research on the Cell Proliferation of Mouse Fibroblast Derivation L929 on Globular Titanium Implant Material

Abstract

There are two methods of evaluating the cell compatibility of metal implant materials using cultured cells: the direct contact method, and the extraction method. In the former, a material is brought into contact with cells directly in a medium, while extract of a material is liberated on the cells in the latter method.
This study was conducted employing the two methods, using globular titanium powder, mouse fibroblast-derived L929 cells, and artificial saliva solution with titanium after anodic polarization in the solution. The results for the adhesion of cells and the cell proliferation characteristics were compared. The results using the direct method showed adhesion of the cells to the globular titanium powder, and the cells grew vigorously three to nine days after the adhesion. Also, adhesion of the cells to the artificial saliva solution was observed, and the cell proliferation after the adhesion was almost the same. The ion elution in this case was measured as 0.8 ppm by inductively coupled plasma mass spectrometry (ICPS).
Using nickel standard solution, which is a positive reference material cell, adhesion and proliferation of cells were not observed.
These results suggest that globular titanium material is suitable for biomaterial because cells adhered to it from the beginning and proliferated.