Baculoviral Inhibitor of Apoptosis Repeat-containing Gene 6 Expression in Osteoblasts on Fibronectin Derived Peptide Coated Titanium

Abstract

The enhancement of adhesion and migration of osteoblastic cells on titanium surfaces is an important process for successful implant therapy. In this study, osteoblastic cells, MC3T3E1, were cultured on GRGDSP peptide coated titanium surfaces. After 15 days of cell culture, total RNA was isolated from cells, and gene expression levels were analyzed by the Gene Chip system. Many gene expression levels were found to be altered in MC3T3E including a baculoviral inhibitor of apoptosis repeat-containing gene 6 (Birc6). The enhancement of Birc6 mRNA levels was successfully confirmed by reverse transcription polymerase chain reaction (RTPCR) and real-time PCR. Since it has been reported that titanium stimulates apoptosis in osteoblasts, these findings suggest that GRGDSP coated titanium may promote cell proliferation of osteoblasts through Birc6 expression.