Original
In Vitro Evaluation of Titanium Surface Functionalization with H2O2 Hydrothermal Treatment and FGF-2
2016 Volume 29 Issue 2 Pages 95-103
Details
2016 Volume 29 Issue 2 Pages 95-103
The present study aimed to demonstrate the influence of surface treatment of fibroblast growth factor (FGF)-2-loaded TiO2 film by H2O2 hydrothermal treatment on in vitro bone bioactivity.
The results suggested that H2O2 hydrothermal treatment resulted in TiO2 films with a lower contact angle and higher hydrophilicity. Early cell adhesion assay by a scanning electron microscope (SEM) suggested that the FGF-2-loaded surface had a high cell density and a significantly higher cell average adhesion area, as well as marked cell extension when compared with the non-FGF-2-loaded surface. MC3T3-E1 osteoblast-like cell proliferation tended to increase with time, with particularly high cell proliferation observed on day 7 and day 14. The alkaline phosphatase (ALP) activity was significantly higher in the FGF-2-loaded group when compared with the nonFGF-2-loaded group.
In conclusion, the results of this study showed that H2O2 hydrothermal surface treatment of FGF-2-loaded TiO2 films can increase early cell adhesion, proliferation and bone differentiation, and contribute to functionalization of the titanium surface, early bone-titanium implant integration and long-term stabilization.
