2019 Volume 32 Issue 4 Pages 339-345
One of the risk factors of implant treatment is patients with diabetes mellitus, as it is difficult to acquire osseointegration compared with healthy patients. There are time-related changes to the titanium surface, and the cell affinity and histocompatibility of the titanium decreases. Ultraviolet (UV) treatment to titanium is known to effectively improve these changes. Therefore, in this study, we investigated whether implant treatment for diabetic patients could be enhanced by UVtreating titanium surfaces cultured with bone marrow-derived osteoblast-like cells of type 2 diabetic rats (Spontaneously Diabetic Torii : SDT). These cells were cultured in a low glucose environment similar to the state in which the blood glucose level was controlled.
The experiment was divided into two groups : 1) The control group in which osteoblast-like cells of SDT rats were placed on control discs and 2) the UV group in which osteoblast-like cells of SDT rats were placed on UV-treated discs. In this study, these osteoblastic cells were derived from SDT rat bone marrow which was cultured in a low glucose environment, and placed onto both the control and UV treated discs. The control discs were used 4 weeks after surface acid-etching treatment. The UV-treated discs were subjected to 48 hours of UV irradiation. In addition, WST-1 Cell Counting Kit, alkaline phosphatase (ALP) activity and Alizarin red staining were used to evaluate t he number of attached cells, differentiation, and mineralization, respectively.
In the UV group, the number of attached cells was small compared with the control group. The ALP activity of the UV group was higher than that of the control group. Furthermore, calcification activity of the UV group was higher than that of the control group.
Collectively, our data suggest that the osteoblast-like cells of SDT rats on the UV-treated discs enhanced differentiation to osteoblasts. It is considered that UV treatment of titanium is a useful method for implant treatment of diabetic patients. However, further study is required to examine the differentiation of osteoblasts and expression of bone formation related genes in a high glucose environment, in addition to further in vivo research of implants for diabetic rats.
