1992 Volume 5 Issue 2 Pages 305-309
Little is known about the cytotoxic effects of Ti-Ni alloys. This study investigated the cytotoxicity of Ti-Ni alloy using the millipore filter method. Human epithelial cells (HeLa) suspended in MEM growth medium to a final concentration of 1.5×105 cells per ml were used. Millipore filter discs with a diameter of 47 mm and pore size of 0.45mm were covered with 6 ml of cell suspension and incubated for 24 hours at 37℃ in a humidified atmosphere of 5% CO2 in air. Test materials comprised of Ti-Ni,Ni,Ti,and asbestos, 7 mm in diameter which were 3.5 g or less in weight. The test procedures followed were those as described by WENNBERG et al. (1979). For control purposes, filters with monolayer of cells,without test specimens were included. Ten specimens for each test situation were used. After incubation, the filter was incubated for cytochemical demonstration of succinate dehydrogenase activity of the cell monolayer (BARKA and ANDERSON, 1963). The control filters were uniformly stained in a dark blue color while the test samples were scored as:(1)Ti-Ni-O (no difference in staining intensity as compared to the control; (2)Ti-O; (3)Ni-I (unstained zone had a diameter less than 7 mm); (4)Asbestos-3(unstained zone was greater than 12 mm wide). Based upon the results of this study, both Ti-Ni and exhibit a non cytotoxic response while,Ni exhibits a mild cytotoxic response and asbestos a markedly cytotoxic response.
