Abstract
Effect of combination with 5-fluorouracil (5-FU) and cepharanthin (CE) on a oral squamous cell carcinoma cell line (KM-1) was examined using flow cytometry (FCM) with DNA/BrdU dual staining. 5-Fu and CE, dosage 1μg/ml respectively, were 5 Fu depressed the DNA synthetic rate until 48 hours after the treatment, and rapid restoration was detected at 72 hours. Combination 5 Fu CE depressed the DNA synthetic rate like 5 Fu until 48 hours. However, at 72 hours after treatment the DNA synthetic rate was observed to be clearly depressed, compared with 5 Fu. It was suggested that CE may suppress DNA synthethis, and the present study confirmed that combination therapy of CE and 5 Fu is effective.
