Abstract
Introduction: The toiletry oral gel, Oralbalance® (Laclede), contains lysozyme, lactoferrin and lactoperoxidase, and has efficacy against bacteria and fungi, as well as good moisture-retaining properties.
Purpose: The purpose of this study is to clarify the antimycotic effect of the Oralbalance® gel on the toiletry of the denture base material surface.
Materials and Methods: Five square mm and 1 mm thin specimens were made from heat curing resin (ACRON: GC), a denture base material. These specimens were soaked in a Candida albicans (ATCC18880) or C. glabrata (ATCC90030) suspension adjusted to an OD5300.5 for 120 minutes, and then washed 3 times with PBS. In the OB group, the specimens were first coated with 25mg of Oralbalance® gel and in the Control group, the specimens were coated with 25mg of petrolatum, then 2 ml of artificial saliva was added per minute near the specimen using a micro pump for 30 minutes. Each specimen was then washed with 1 ml of PBS 3 times and soaked in 1 ml of 1% of TRITON 100X® (ROSCH) for 10 minutes. Then, 10 microliters of the soak solution was cultivated on CHROMagarTM Candida (B & D Japan) for 48 hours, and the colony forming units (CFU/ml) were counted. Each specimen was observed by low-vacuum scanning electron microscopy (HITACH Miniscope® TM-1000).
Results: The CFU/ml of the OB group was significantly lower (P < 0.01, unpaired t-test) than the CFU/ml of the Control group. There were many yeast and or hyphae in the microgrooves or micro pits on the specimens from the Control group, but little or no yeast and/or hyphae on the OB group specimens.
Conclusion: The toiletry oral gel, Oralbalance®, may have an antimitotic effect on the denture material surface.
