Abstract
In the present study, collagen pellets containing minocycline (M-P) and lysozyme chloride (L-P) were examined for the slow release of drugs and their effects on inflammatory symptoms. Testing of slow release was conducted using the UV measurement method (in vitvo) and HPLC (in vivo) for M-P and bioassay for L-P. Clinical examination (GI, BOP, and SI) as well as determination of lysozyme chloride concentration and the amount of gingival crevicular fluid (GCF) were performed in 12 patients with adult periodontotitis before, and 1, 3 and 7 days after administration of L-P or oral Leftose®. Seventeen patients with adult periodontitis receives M-P alone or in combination with L-P. The minocycline concentration and amount of GCF were determined together with the clinical examination in these patients before, and 3 and 7 days after administration. The results showed that L-P provides lower drug release rates than M-P. The lysozyme chloride concentration in periodontal pockets increased on days 1 and 3 of administration in the L-P group, and on days 3 and 7 in the oral Leftose® group. Both the concentration and percent residue of minocycline in periodontal pockets were higher in the combined M-P and L-P group than in the group that received M-P alone. Clinical symptoms of inflammation improved more in the L-P group than in the oral Leftose® group, and in the combined M-P and L-P group than in the group that received M-P alone. GCF also decreased significantly in these groups over time. These results indicate that inflammatory symptoms are improved soon after local L-P administration and that the retention of minocycline in periodontal pockets is elevated after M-P administration
