Abstract
In the 1960s, Seeman et al, reported the effects of local anesthetics on the cell membrane, stating that low concentrations of local anesthetics strengthen the erythrocyte membrane and exert an antihemolytic effect as a result, but high concentrations of them cause hemolysis. There have been other reports concerning the interaction between anesthetic potency and hemolysis. In this study, we determined the antihemolytic effect of local anesthetics in human erythrocyte membrane and their correlation to anesthetic potency. Each local anesthetic was added at various concentrations (0-10mM) to isotonic TRIS. LA isotonic TRIS was added to 75μL of erythrocyte solution. Subsequently, hypotonic TRIS which had the same concentration of local anesthetic as the LA isotonic TRIS, was added. The resultant solution was centrifuged at 12, 000g for 5 min. The absorbance of the supernatant to a wavelength of 540nm was measured using a spectrophotometer. The antihemolytic effect of the local anesthetic at each concentration was obtained by the following formula: (Absorbance of local anesthetic at concentration of 0 mM absorbance of local anesthetic at each concentration [0.1-10mM] ) / (absorbance of a local anesthetic at concentration of 0 mM) .
Procaine did not show at express antihemolysis effect. Lrdocaine indicated an antihemolysis ef-fect of 0.4mM and between 0.8 and 10mM between the control (p<0.05) . Prilocaine indicated an antihemolysis effect of 2 to 10mM between the control (p<0.05) . Mepivacaine indicated antihe-molysis effect of 2 to 4 mM between control (p<0.05) . Articaine indicated an antihemolysis effect of 0.6mM to 10mM between the control (p<0.05) . Based on these results, if an anesthetic has a direct action on nerve fiber, anesthetic potency is in the following order: lidocaine≥articaine>mepivacaine=Prilocaine.
