Abstract
Back ground: Mucosal changes associated with food-sensitive intestinal inflammation have been identified in several animal model mice. The histological examination will provide an important clue for clarifying the mechanism of food hypersensitivity. However it is unknown where the severest inflammation occurs.
Method: To identify the severest inflammation site, we focused on the histological changes in Nc⁄Jic mice sensitized with ovalbumin (OVA). We sensitized Nc⁄Jic mice (n=7) with low doses of OVA both orally and peritoneally, and then sacrificed 3 hours after a high oral dose of OVA challenge. The control group (n=7) was primed with saline in the same manner. The severity of mucosal inflammation, the villous edema occurrence, and the number of intramucosal mast cells of the OVA group were compared with those of the control group in the entire small intestine, 3 parts and 30 parts divided equally.
Results: The severity of inflammation and the villous edema occurrence of the OVA group were significantly higher than those of the control group in the pyloric and central parts, and many of the significantly higher segments were found in the pyloric part. The number of mast cells was significantly lower in the pyloric part of the OVA group than that of the control group.
Conclusion: Food hypersensitivity mucosal inflammation is the severest in the pyloric part.
