Abstract
In cases of traumatic tooth avulsion, the existence and the integrity of periodontal ligament (PDL) cells play key roles in success of the replantation. We evaluated the effects of different types of commercially available tooth storage medium on human PDL cells in regard to cell viability, morphology and changes in pH of the medium.In this study, human PDL cells were placed in Teeth Keeper NEO (TN), DentSupply (DS), physiological saline, physiological saline added HEPES, cell culture medium, and cell culture medium added HEPES for 30 minutes and 3, 6, 12, and 24 hours at room temperature. The number of viable cells at those time points was determined using a colorimetric assay of dehydrogenases in the storage medium. The highest level of cell viability was observed in cell culture medium with HEPES, which was used as a positive control. The rapid reduction of cell viability in cell culture medium was rescued by adding HEPES, indicating that the reduction was caused by pH changes during the experiment. Cell morphology findings also showed the least degeneration in the medium. The cell viability and integrity in TN and DS were maintained for up to 6 hours, and then gradually decreased with time. There were no significant differences between TN and DS in regrard to cell viability throughout experimental period, though PDL cells in TN showed less degeneration at 12 and 24 hours in terms of cell morphology.
