Abstract
Glucose, an essential source of energy for determination of size and form during tooth development, is transported across the cell membrane by glucose transporters (GLUTs), while epigenetic alterations are crucial events for tissue development and histone acetylation is a major epigenetic event that is regulated by histone deacetylases (HDACs). Although epigenetic events may occur in the process of tooth development, the involvement of epigenetic modifications remains unclear. We investigated immunohistochemical localization of GLUTs and HDACs during tooth development in mice to determine their effects on tooth development. Dental germ tissues were subjected to immunohistochemical staining with anti-GLUTs and anti-HDACs, and PAS staining was also performed. HDAC3 and GLUT 1 were found to be co-localized in the enamel organ and mesenchymal tissue surrounding the tooth germ in the cap stage. In addition, localized HDAC5, GLUT3, and PAS staining was seen in the dental lamina, mesenchymal tissue surrounding the enamel organ, and dental papilla in the cap stage. In the early bell stage, HDAC3 and GLUT1 were co-localized in the stellate reticulum, dental papilla, and mesenchymal tissue, while HDAC5, GLUT3, and PAS staining was observed in the dental lamina and mesenchymal tissue. No expression of HDAC5 was seen in the bell stage. Our results suggest that GLUT and HDAC expressions, and their cooperation play critical roles in tooth development.
