Abstract
The purpose of this study is to determine if pep t i des which relate to carious susceptibility patterns and activity, can be binded in saliva of the children with decayed teeth by SDS-polyacrylamide gel electrophoresis. The subjects were 71 children,3 to 10years of age. In first examination carious susceptibility patterns was recorded, carious activity and was tested,2 ml of whol oral saliva were collected. Saliva was added to 80% methanol and lyophyrized as the starting sample to be used. These samples were electrophoresised using SDS-polyacrylamide gel.
The following results were obtained.
1) The salivary samples were divided into about 5 to 20 bands using SDS-polyacrylamide gel electrophoresis, we discovered that these bands were differen t according to each subject.
2) In the relationship between carious susceptibility patterns and salivary peptides, we were able to discover that carious type IV saliva has considerably fewer peptide ban ds than the other types.
3) In the relationship between carious activity and salivary peptides, we found particuler bands, namely, peptides (molicular weight 50,000 to 100,000) were found in lower carious activity saliva, and peptides (molicular weight 1,500 to 4,500) were found in higher carious activity saliva, and these peptides included much prolin.
