Abstract
Two kinds of metal specimens which were commercially available deciduous crowns were used for experimental study, Ni and Cu were evaluated their toxicity against fibroblasts, using the tissue culture technique.
The cell line of the fibroblasts was established, which were derived from human gingiva.
Fibroblasts were prepared as 1×105 cells per 1 ml of culture media, which consisted of Eagle MEM with 10% bovine calf serum. Metal specimens were cut in sections of 6×6×0.2 mm3, which were put in petri dishes (φ60×15mm).5ml of cell suspension was put into the same petri dishes. The culture dishes were placed in incubator. The incubator was adjusted to a temperature of 37°in humid atmosphere, and 5% CO2 was fused into the incubator. These petri dishes were kept in the incubator for 7 days.
Cell numbers were counted from the neighborhood of the metal specimens on the 1st,2nd,4th and 7th experimental days in order to investigate the biocompatibility between the metals and fibroblasts.
The results were as follows:
1) Control cultured fibroblasts were grown nicely without metal specimens. The cells were counted as 11.44×104,23.55×104,53.71×104, and 60.00×104cells/ml on the 1st,2nd 4th and 7th days respectively.
2) The cultured fibroblasts with the metal specimens, which were made from commercially available deciduous crowns A and B were grown nicely as well as the controls. Between these two studies, no sigificant differences were investigated (P<0.01).
3) The fibroblast cells with the Ni-specimen were grown differently from the control studies. The cells were counted as 6.88×104,13.73×104,32.33×104and 45.18×104 cells/ml on the 1st,2nd,4th and 7th experimental day srespectively. These were significantly different (P<0.01) from the control studies. Dead cells around the Ni-specimens were observed and the mitotic index was diminished gradually every day.
4) The fibroblast cells with the Cu specimen were grown slowly until the 2nd day and the mitotic activity was very poor. No living cells were observed on the 4th day and the cells were very much atrophic and degenerated, as far as their morphology are concerned.
As a result of these experimental studies, two kinds of deciduous metal crowns were revealed to show good biocompatibility against the fibroblast cells, which were derived and establshed from male human gingival tissue.
