Abstract
Lately, investigations at the cell level of bone resorption and ossification have been very evident. This is due largely to the technical advances made in 'cell culture. ' Investigations of the characteristics of osteoblasts from the first generation culture of the jaw bones, however, have been extremely few in number.
With a view to making a comparative study of the characteristics of osteoblasts of different derivations, the authors obtained, by the enzyme digestion method, osteoblasts from the mandible and those from the calvaria of rats at the lactation stage during which mechanical stress was considered minimal.
The following, measurements were then taken:
1) ALp stain,2) ALp activity,3) volume of protein,4) ALp activity per volume of protein. The results were as follows: 1) With the ALp stain, mandible-derived osteoblasts showed higher stainability than calvaria-derived osteoblasts.
2) In the ALp activity, mandible-derived osteoblasts showed an average of 0.572±0.094 nmol/ul and calvaria-derived osteoblasts 0.288±0.044 nmol/ul. In the t-test, mandible-derived osteoblsts showed significantly higher values (p <0.01) than calvaria-derived osteoblasts
.3) In the volume of protein, mandible-derived osteoblasts showed an average of O.353±0.080 ug protein/ul and calvaria-derived osteoblasts 0.312±0.040ug protein/ul. In the t-test, no significant differences were found between the two.
4) In the ALp activity per volume of protein, mandible-derived osteoblasts showed an average of 1.636±0.140 nmol/ug Protein and calvaria-derived osteoblasts showed significantly higher values (p <0.01) than calvaria-derived osteoblasts.
