Abstract
The purpose of this study was to establish a new DNA extract method in humans the toothbrush. This method is considered to be one of the most useful approachs in the dental area because it confines the damage to the minimum against body injury and does not cause the patient any anxiety. The following are the results of the present investigation.
(1) A high molecular weight DNA was obtained from all of the patients tested.
(2) The level of the amount of extracted DNA from the tongue was significantly higher than that of the gingival and mucousmembrane site.
(3) For the amount of DNA recovered, it was shown that with 10 strokes were much more was recovered than with 1 stroke on each site in this study.
(4) Using the primer which responded especially to human DNA, we were able to amplify the tested samples using the polymerase chain reaction.
We concluded that the new DNA extraction method using tooth brush could be used as a useful tool to study the dentition abnormalities caused by gentincal factors such as conginital missing, teeth fused teeth and supernumerary teeth. Key words: Toothbrush, Human DNA extraction method, Polymerase chain reaction (PCR)
