Abstract
When a tooth is avulsed by trauma, some form of storage should be undertaken to prevent the drying of the periodontal ligament. Milk has been recommended as the solution for use with avulsed teeth caused by trauma. At present, solutions of two types for use as preservation solutions for extracted teeth are on the market in Japan. One was made for long term preservation of a implanted or transplanted teeth, while the other was made for short term preservation of avulsed teeth.
The purpose of this study was to compare the viability of cultured human PDL cells in the storage medium.
Three solutions including milk, preservation solution for dental implanted or transplanted tooth (Solution D), and a preservation solution for avulsed teeth (Solution N) were used as experimental solutions in this study. MEM including 10% FCS was used as the control media. The PDL cells were cultured from healthy extracted human teeth.
After the PDL cells were cultured for 8 days, they were exposed to each solution for 15 minutes at 4°C. The MTT assay was measured immediately after exposure and subsequently followed at 1,2,3,5 and 7 days after exposure. The morphological changes in the PDL cells were observed immediately and on 7 th day after exposure.
The results were as follows.
1. The growth activity: Milk and Solution N always showed superior activity. Solution D revealed poor activity from immediately after exposure to 7 days after exposure.
2. The morphological changes: Milk and Solution N caused the appearance of healthy cells with spindle-like morphology. In Solution D, at immediately after exposure, the cells had appeared a poor morphological appearance, and cells detached themselves from the plate on 7 days after exposure.
These results suggest that Solution N is as effective as same milk. and Solution D is ineffective for PDL cells.
