The Japanese Journal of Pediatric Dentistry
Online ISSN : 2186-5078
Print ISSN : 0583-1199
ISSN-L : 0583-1199
Effect of Calcium Hydroxide on Cultured Human Diploid Fibroblasts
Ayuko Okamoto, Tomomi Kawakami, Yoshiyuki Ohide, Mayuri Kaneko, Takako Tsunoda, Kazuhiko Ogihara
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Keywords: Calcium hydroxide, Human diploid fibroblasts, Calcium ion
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1999 Volume 37 Issue 1 Pages 62-68

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Abstract
The cytotoxicity of calcium hydroxide (Ca(OH)2) on human diploid fibroblasts (WHE-7 cells) and human gingival fibroblasts (GFB cells) was examined. The growth of WHE-7 cells and GFB-cells was not suppressed by treatment with Ca(OH)2 at 0.003%-0.03% for 24-72h. The cytomorphological changes of both fibroblasts with Ca(OH)2 were not microscopically observed. The concentration of calcium ion (Ca2+) from calcium hydroxide at 0.003%-0.03% for 72 h were increased in a dose-related manner. That of calcium ion in the cell on the other hand did not change.
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© The Japanese Society of Pediatric Dentistry
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