The Japanese Journal of Pediatric Dentistry
Online ISSN : 2186-5078
Print ISSN : 0583-1199
ISSN-L : 0583-1199
Cultured Human Periodontal Ligament Cells Produce Nitric Oxide Through the Interleukin-1β-independent mechanism
Takashi KikuiriTetsuo ShirakawaTomokazu HasegawaYoshitaka YoshimuraSadaaki TakeyamaMasayuki KagaHaruhisa Oguchi
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1999 Volume 37 Issue 4 Pages 768-774

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Abstract
Nitric oxide (NO) has been implicated as a biological mediator in a number of organs. We found that cultured human periodontal ligament (hPDL) cells spontaneously produce NO in a serum-free condition. This NO production was not affected by culturing the cells with interleukin-1β(IL-1β). The amount of NO production in the hPDL cells (1.0×106 cells/well) was 0.28±0.23 nmol/12 h when the cells were cultured with a serum-free medium and was 0.41±0.22 nmol/12 h with a medium containing 20 ng/ml IL-1β. In contrast, NO production in the mechanically stimulated hPDL cells(4.0×105 cells/well)was 9.22±1.37nmol/12h in αMEM containing 10% fetal calf serum. The value was significantly higher (p<0.001) than the control value (1.04±0.22nmol/12h) obtained from unstimulated cells. The NO production was dependent on both magnitude of the tension force and frequency of the stretching cycle.
These results indicate that hPDL cells produce NO independently of IL-1β and that the NO production was enhanced by mechanical stimuli.
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© The Japanese Society of Pediatric Dentistry
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