Abstract
The purpose of this study was to investigate the affect of b-FGF and TGF-βon the infant human alveolar process of the maxilla-derived osteoblastic cells(HAB). The result were as follows.
1. While b-FGF(2.5 ng/ml) and TGF-β (0.5 ng/ml) stimulated the cell proliferation of HAB, the ALP activity was inhibited.
2. While TGF-β(1.0 ng/ml) inhibited the cell proliferation of HAB, the ALP activity was stimulated.
3. TGF-β(0.5 ng/ml)+b-FGF(2.5 ng/ml) further stimulated the call proliferation of HAB, the ALP activity was further inhibited.
4. While b-FGF(2.5 ng/ml) stimulated the expression of osteocalcin mRNA in HAB, it did not markedly effect expression of ALP activity and Type I collagen in HAB.
5. While TGF-β(1.0 ng/ml) inhibited the expression of osteocalcin mRNA in HAB, it did not markedly effect expression of ALP activity and Type I collagen in HAB.
The results indicated that b-FGF and TGF-βregulate cell growth and differentiation in the infant human alveolar process of the maxilla-derived osteoblastic cells(HAB).
