Abstract
Knowing the incidence of HSV-1 positive rate is important for pedodontist to undertake diagnosis, treatment and prevent of herpetic gingivostomatitis that is manifested severely in some cases.
No report investigating the incidence of HSV-1 in latent infection using a more improve detected method than the usual PCR in saliva has been.
The Nested PCR-Real-time PCR method was first established as a method of high sensitivity and detection of the Herpes Simplex Virus type 1 (HSV-1) by the authors. Samples of saliva were collected from 253 healthy children 3 to 12 years of age. All of the DNA was extracted from the saliva and the DNA of HSV-1 was amplified using the nested PCR method using the Real-time PCR.
The results were as follows:
1. Herpes simplex virus type 1 (HSV-1) in saliva increased for ages 3 to 12 in healthy children.
2. The positive HSV-1 was detected in around 85% of the saliva samples in aged children 12 years of age and 27% of the saliva samples in children 7 years of age by the nested PCR - Real-time PCR method.
It was suggested that the majority of primary infection of HSV-1 has changed from the period of infancy to schoolchildren, and most humans experience the primary infection of the virus until the age of 12.
