Effect of Calcium Carbonate on Alkaline Phosphatase Ability of Human Dental Pulp Cells

Abstract

The purpose of this study was to investigate the effects of calcium carbonate (CaCO₃) on the mlneralization ability of human dental pulp (HDP) cells. HDP cells were treated with 1mM,100μM,10μM and 1μM of CaCO3 for up to 12 days. Non-treated cells served as a control. The cytotoxicity effeet on HDP cells were not detected by treatment with each concentration of CaCO₃. The cell productivity of HDP cells was highest in cultures treated with 10μM-CaCO₃ group. We compared the alka-, line phosphatase (ALP) staining and ALP activity of treatment with each concentration of the CaCO₃groups and control. A larger number of the 10μM-CaCO₃ groups showed positive with ALP staining than other groups. The ALP activity was also highest in cultures treated with 10μM in the CaCO3groups. These results suggested that the mineralization ability of HDP cells was enhanced by treatment with 10μM in the CaCO3 groups.