Visualization of ATP dynamics during mouse exercise

Abstract

Muscle contraction requires energy. However, the dynamics of adenosine triphosphate (ATP) in the skeletal muscle contraction in vivo is unknown. Therefore, we tried to visualize ATP dynamics in mice. By performing various gene modifications using Goateam reported as FRET-type fluorescent protein that senses ATP, we succeeded in creating a mouse that can visualize ATP level in the cytoplasm. By resting the skeletal muscle of this mouse from contraction exercise, it succeeded in capturing in vivo the decrease in the amount of ATP by exercise and the recovery of the amount of ATP by rest. This mouse can be used to evaluate muscle movement in vivo by ATP dynamics.