局外生規ジョテイシの確認試験の設定と指標成分の同定について

丸山 卓郎; 吉冨 太一; 西尾 雅世; 中川 和也; 横倉 胤夫; 山本 豊; 神本 敏弘; 近藤 誠三; 内山 奈穂子; 白鳥 誠; 田辺 章二; 土屋 久美; 中田 孝之; 若林 健一; 高尾 正樹; 高橋 喜久美; 松本 和弘; 武田 修己; 嶋田 康男; 佐々木 博; 川原 信夫; 袴塚 高志

doi:10.24684/jspharm.73.2_84

Natural Resource Letter

Design of Identification Test for Ligustrum Fruit on Non-JPS and Identification of Its Marker Compound

Takuro Maruyama, Taichi Yoshitomi, Kayo Nishio, Kazuya Nakagawa, Tsuguo Yokokura, Yutaka Yamamoto, Toshihiro Kammoto, Seizo Kondo, Nahoko Uchiyama, Makoto Shiratori, Shoji Tanabe, Kumi Tsuchiya, Takayuki Nakada, Ken-ichi Wakabayashi, Masaki Takao, Kikumi Takahashi, Kazuhiro Matsumoto, Osami Takeda, Yasuo Shimada, Hiroshi Sasaki, Nobuo Kawahara, Takashi Hakamatsuka

Author information

Takuro Maruyama
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Taichi Yoshitomi
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Kayo Nishio
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Kazuya Nakagawa
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Tsuguo Yokokura
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Yutaka Yamamoto
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Toshihiro Kammoto
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Seizo Kondo
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Nahoko Uchiyama
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Makoto Shiratori
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Shoji Tanabe
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Kumi Tsuchiya
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Takayuki Nakada
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Ken-ichi Wakabayashi
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Masaki Takao
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Kikumi Takahashi
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Kazuhiro Matsumoto
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Osami Takeda
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Yasuo Shimada
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Hiroshi Sasaki
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Nobuo Kawahara
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)
Takashi Hakamatsuka
Working group for Non-JP Crude Drug Standards 2018 (Non-JPS 2018)

Keywords: Ligustrum Fruit, identification test, TLC, nuzhenide

JOURNAL FREE ACCESS

2019 Volume 73 Issue 2 Pages 84-88

DOI https://doi.org/10.24684/jspharm.73.2_84

Details

Abstract

Ligustrum Fruit is a crude drug derived from the fruit of Ligustrum lucidum W.T. Aiton or L. japonicum Thunb. (Oleaceae). It has several pharmaceutical activities including hepatoprotection, antioxidation and improvement of bone turnover and therefore, it is used as an ingredient in many crude drug products for nutrition fortification. In preparation for the listing of the drug to Non-JP Crude Drug Standards (Non-JPS), we designed an identification test using TLC and identified the marker spot as nuzhenide based on the spectroscopic data including ¹H-, ¹³C-NMR and MS together with the comparison of TLC, LC-MS and NMR with those of the authentic compound.

The established TLC conditions are as follows: chromatographic support, silica gel; developing solvent, EtOAc/MeOH/H₂O (7/2/1); developing length, 7 cm; detection, UV (254 nm) and 1-naphthol-sulphuric acid reagent; R_f value, 0.4 (nuzhenide).

Corresponding author

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