Abstract
Multiprotein bridging factor 1 (MBF1) had been identified as a transcriptional coactivator that bridged between the TATA-box binding protein (TBP) and the DNA binding regulatory factor BmFTZ-F1 in silkworm. MBF1 homologs are conserved from yeast to human. However, it is largely unknown about the function of plant MBF1 homologs.
By differential display approach, we isolated independent 38 cDNA clones that were specifically expressed in calli derived from roots of Arabidopsis thaliana. One of them was a MBF1 homolog. We therefore designated it AtMBF1a. Furthermore, additional two MBF1 homologs, AtMBF1b and AtMBF1c, were found in the A. thaliana genome.
In this report, we analyzed the expression patterns of these three genes in Arabidopsis. Northern blot analysis revealed that these were expressed in various organs, especially in flowers. Furthermore, their promoter-GUS transgenic plants were constructed and these histochemical analysis suggested that AtMBF1c was involved in response to wounding.
