Abstract
The dioecious plant, Silene latifolia has chromosomal distal end specific satellite DNA (Sl-distal-satDNA). Eleven BAC clones containing this satellite DNA were isolated from a partial BAC library of S. latifolia. They were digested with six restriction enzymes and hybridized with the Sl-distal-satDNA as a probe. This Southern blot demonstrated that they contain the Sl-distal-satDNA showing typical ladder patterns of repeated DNA. A phylogenetic tree was constructed using the neighbor-joining algorithm to investigate pair-wise similarities between individual repeat units of the Sl-distal-satDNA. The repeat units derived from eleven BAC clones formed four clusters named as SacI, KpnI, E and F family, respectively. Multi-colored FISH with the SacI family and the KpnI family probes demonstrated that the KpnI family is localized at only one end of chromosome 7. These results suggest that there is nucleotide diversity among individual families of the Sl-distal-satDNA.
