Abstract
ABI3, LEC1 and FUS3 are thought to be major transcriptional activators expressed specifically during seed formation. Deficient mutants of the seeds show the reduction of storage proteins, which are classified into late embryogenesis abundant (LEA) proteins. We had identified embryogenic cell proteins (ECPs) from carrot, and isolated the corresponding genes (DcECP31, DcECP63). These genes are regulated by carrot ABI3 homolog, C-ABI3, and expressed in the lately stage of seed development. However, in carrot, FUS3 gene homolog has not isolated yet, and there are no deficient mutants of C-ABI3, C-LEC1. We had already isolated Arabidopsis homolog to DcECP31 and DcECP63 (AtECP31 and AtECP63) and are examining the expression pattern. Using deficient mutants and transgenic plants, we clarified that factors controlling expression of AtECP31 were different from those of AtECP63.
