Abstract
Snare proteins lie at the heart of membrane fusion events in the secretory and endocytic pathways. Development of root nodules on legume plants following interaction with Rhizobium involves a series of unique cellular and molecular events. The nature of this newly formed subcellular compartment and the targeting mechanism to this structure are not known. In order to dissect the process of nodule specific membrane biogenesis, we have screened 12 SNARE-like clones that have high homologies to SNARE genes of yeast from EST clones of Lotus japonicus. Northern blot analysis revealed that two genes expressed at high level in nodule. These two genes are high homology to sed5 gene of yeast. In situ hibridization showed that these genes are localized at the non-infected cells. We try to find the immuno localization of these proteins.
