Abstract
DREB1 is a transcription factor that binds to a drought, salt and low-temperature responsive element, DRE and induces various functional genes that function in stress tolerance in Arabidopsis thaliana. Expression of the DREB1 gene family, DREB1A, 1B and 1C are rapidly induced by low-temperature stress, and at least six motifs are conserved in all three promoters. Promoter analysis of the DREB1C gene showed that 67 bp region from -113 to -47 in the DREB1C promoter was sufficient for low-temperature responsive expression of DREB1C. Two of conserved six motifs among three DREB1 promoters, Box-V containing a G-box and Box-VI were included in this region. Furthermore, gel mobility shift assay revealed that the 67 bp region formed a complex with nuclear extract prepared from non-treated or low-temperature-treated Arabidopsis. Currently, we perform yeast one-hybrid screening uning cDNA library prepared from non-treated Arabidopsis plants.
