Abstract
A 507-bp sequence (designated TJ1) of a nuclear matrix attachment region (MAR) cloned from a transgene locus of tobacco cells was flanked in four different orientations to the 5' and 3' ends of the expression cassette of the green fluorescent protein (GFP) gene. In each construct, the GFP gene was under the control of the cauliflower mosaic virus 35S promoter and the nopaline synthase terminator. These constructs were introduced separately into tobacco cells, and the yield of stable transformants was determined. At the most more than 2 times increase in the yield was obtained by the presence of TJ1-MAR in the expression cassette. It is thus concluded that TJ1 increases transgene integration efficiency.
