Abstract
Intercellular communication of plant cells is held by plasmodesmata (PD). PD form cytoplasmic continuity and some macromolecules move through them. PD components and mechanism of macromolecule transport are still unclear. On the other hand, plant viruses utilize PD as paths to invade cells. Movement protein (MP) of tobamovirus is localized in PD, increases the size exclusion limit.
It is likely that MP interacts with PD proteins, which are involved in macromolecule transport. To identify such proteins, we analyzed cell wall/PD proteins co-extracted with MP using transgenic tobacco expressing MP. After treatment with salt in high concentration, MP was extracted. 24K and 25K proteins were major components of the MP-enriched fraction. N-terminal amino acid sequences of them indicate that they were germin like proteins (GLP). One of them has amino acid sequences homologous to peach auxin-biding protein 19/20 and to barley HvGLP1.
