Plant and Cell Physiology Supplement
Supplement to Plant and Cell Physiology Vol. 44
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DNA methylation in the transgenic tobacco plant exhibiting silencing of the ω-3 fatty acid desaturase gene.
*Kouta TakahasiTomomi AbikoMasahiro MasadaHiroaki Kodama
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Pages 282

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Abstract
Tobacco microsomal ω-3 fatty acid desaturase (NtFAD3) catalyzes conversion of linoleic acid into α-linolenic acid. Most of transgenic tobacco plants with the NtFAD3 gene showed increased linolenate level. One of the overexpressing line is named S24. On the other hand, a transgenic line named S44 exhibited a lower level of leaf linolenate than the wild-type plants. In the S44 leaf tissues, expression of both transgene and endogenous counterparts was suppressed post-transcriptionally. In the most post-transcriptional gene silencings(PTGS), the coding region of a transgene is often methylated. Here we investigated the methylation pattern of the NtFAD3 transgene and the enodogenous NtFAD3 gene in the S44 plants. The plant DNA was digested by methylation-sensitive restriction enzymes and then the region containing the restriction-enzyme recognition site was amplified. The amplified pattern indicated that the transgene in the S44 plant was hypermethylated. To characterize the methylation site in more detail,bisulfite analysis is under investigation.
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© 2003 by The Japanese Society of Plant Physiologists
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