Analysis of Two Cool Temperature-responsive Genes in Rice Anther, which are Engaged in Synthesis of Jasmonate and Polyamine

Abstract

In northern part of Japan, rice crop production is occasionally damaged severely by low temperature in summer due to the disturbance in pollen formation. Using cDNA microarray system, we analyzed rice anthers cooled at the young microspore stage, which is the most cool temperature-sensitive stage, in order to identify and characterize genes responsive to cool temperature and are involved in cool temperature damage process in rice pollen formation. We found that many genes are up- or down-regulated by cool temperature in rice anther, and among these, a gene for jasmonate biosynthesis were significantly up-regulated and a gene for polyamine biosynthesis were significantly down-regulated, respectively. We will report the gene organization and expression pattern under cool temperature stress in pollen development stage.