Abstract
Cold deacclimation (DA) is a phenomenon that is genetically controlled to lose the degree of freezing resistance acquired during cold acclimation (CA) and is triggered by spring warmth. DA is also accompanied by a restart of plant growth arrested during CA. We hence set out to establish an experimental system of Arabidopsis for studying DA at molecular level, which may enhance our understanding of the regulation of freezing resistance and growth. We demonstrated that an application of the protein synthesis inhibitor cycloheximide at the onset of DA treatment prevented significantly the subsequent loss of freezing resistance, suggesting that novel gene expression is required for the development of DA. We therefore conducted macroarray and differential display analyses to list up genes that showed altered transcript levels during DA. These results provide us with a future guideline for studying DA at molecular level. (Supported in part by a grant from PROBRAIN.)
