Abstract
Peroxisomes are known to possess several metabolic pathways, such as fatty acid β-oxidation, glyoxylate cycle and glycolate metabolism, and play important roles for lipid degradation and photorespiration. We have isolated Arabidopsis mutants that had defect in fatty acid β-oxidation. Of those, we succeeded in identifying three alleles, ped1, ped2 and ped3, that have defects in 3-ketoacyl CoA thiolase, AtPex14p and peroxisomal membrane ABC transporter, respectively. Lipid degradation of all these mutants is reduced because of the termination of fatty acid β-oxidation at a different step in each mutant.
Since degradation of seed reserved lipid in peroxisomes is an important process, defects in fatty acid β-oxidation may affect gene expression ocurring during postgerminative growth. To investigate regulation of the gene expression, we compare gene expression of the mutants. We discuss function of genes that alter their expression in the mutants.
