Abstract
ANgene of Arabidopsis encodes the homolog of the human CtBP which regulates polar elongation of the leaf cells. AN homologs from Marchantia polymorpha(MAN) and Ipomoea nil(MAN) rescued the an-1 mutation in terms of leaf morphology. MAN does not conserve the LXCXE domain which were contained in the AN and IAN. Moreover, although LXCXE domain is known to have ability to interact with Rb, AN does not interact with Rb in Yeast two-hybrid system. Taken together, we concluded the LXCXE domain of AN is not necessary to regulate the leaf width. Another yeast two-hybrid analysis indicated that AN can not interact with Ela, unlike mammalian CtBP. RT-PCR suggested that the transcriptional expression level of AN is important to regulate the leaf width. We analyzed relationship between translational level of AN and the an phenotype. Based on these results, possible role of AN will be discussed.
