Abstract
Reactive oxygen species overproduced under various environmental stresses directly or indirectly cause various chemical modifications of plant proteins. In this study, we detected chemical modifications of proteins in stressed cucumber plants (Cucumis sativus L. suyo) by immunoblotting. Antibodies used for analysis are, anti-AGE antibody, anti-carboxymethyllysine (CML) antibody, anti-pentosidine antibody (recognizing glycation-derived modifications, Dojin), anti-malondialdehyde (MDA) antibody, anti-acrolein antibody (recognizing lipid-peroxidation-derived modifications, NOF Life Science). Oxydative modification of proteins was detected by OxyBlot Protein Oxidation kit (Invitrogen). In results, during 8h of drought stresses treatment, increase of modification by MDA was observed both in soluble and membrane protein fractions. Our previous study showed that chemical modification loses stability and function of proteins in vitro, therefore, increased chemical modification of proteins caused under environmental stresses might be involved in the functional loss of stressed plant cells.
