Abstract
We had isolated PnCRY1 (Pharbitis nil Cryptochrome 1) whose transcripts transiently increased during the 16-h flower inductive darkness in leaves from an absolute short-day (SD) plant P. nil cv Violet, using fluorescent differential display (FDD) method. PnCRY1 encoded a putative polypeptide of 675 amino acids with a molecular mass of 76 kD. The deduced amino acid sequence of PnCRY1 showed 79% and 73% amino acid identity with that of tomato and Arabidopsis, respectively. PnCRY1 mRNA accumulation showed diurnal fluctuation in both SD and long day (LD) conditions. This oscillation showed circadian rhythm during the extended dark period (DD), but damped under continuous light condition (LL). We wish to show the results of its expression analysis in various conditions (different photoperiodic condition, effect of night break treatment, organ specificity, difference of cultivars, etc), and discuss on the participation of PnCRY1 in the photoperiodic induction of flowering in P. nil.
