Abstract
We demonstrated the search for bioactive substances involved in flower bud formation from Arabidopsis thaliana. A. thaliana grown under short day were transfered to long day or kept under short day, and then, they were extracted with methanol. When compared HPLC chromatograms of each extract, clear decreasing peaks were detected in the long day-treated sample. For identifying them, large amounts of A. thaliana were extracted with methanol. The extract was purified by HPLC,and the isolated sample was determined as monogalactopyranosyl diacylglycerol (MGDG). Whether or not this substance causes flower bud formation of A. thaliana was determined. The substance induced flower bud formation of A. thaliana, which was treated to long day for 1day just before onset of its application. These results suggest MGDG as precursors or substrates of flower bud-forming substances. We will also report two new glycolipids, Arabidopsides A and B from flower forming A. thaliana.
